China Animal Tissue Direct PCR kit (without Sampling Tools) Protocol Manufacturer and Supplier

Animal Tissue Direct PCR kit (without Sampling Tools) Protocol

Short Description:

Kit Description: Directly use animal tissue lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.◮No time-consuming and expensive DNA purification is required.◮The sample demand is small, just take a single seed.◮No special treatments such as grinding and crushing are required, and the operation is simple.◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

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Product Detail

Product Tags

Descriptions

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I	Buffer AL
	Protease
	6× DNA Loading Buffer
Part II	2× PCR Easy^TM Mix

Features&advantages

■ No time-consuming and expensive DNA purification

■ The sample demand is small, as little as 5mg animal tissue can be tested.

■ No special treatment such as grinding and crushing is required, and the operation is simple.

■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit parameters

Application: a variety of animal tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Work flow

Storage and Shelf life

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×PCR Easy^TM Mix can be stored for 12 months at -20℃ ; if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).