The Flooring Expert
Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix for Genotyping of Blood
Specifications
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTM Mix(UNG)-EDTA :On the basis of 2×SuperEasyTM Mix-EDTA, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added at the same time, which can effectively prevent the contamination of PCR amplification products. Contains the specially modified D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. In the PCR reaction, just add the appropriate anticoagulated whole blood, primers, and ddH2O to the corresponding 2× SuperEasyTM Mix(UNG)-EDTA to be used in the PCR reaction.
6× DNA Loading Buffer：The Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer included with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of bright light in the lane during electrophoresis, which will affect the experimental results.
Kit components
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2× SuperEasyTM Mix(UNG)-EDTA |
| 6× DNA Loading Buffer |
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Instructions |
Features&advantages
- There is no need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
-The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
-2× SuperEasyTM Mix(UNG)-EDTA has high blood tolerance: up to 45% in human blood and 20% in mouse blood.
-The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix(UNG)-EDTA, effectively eliminates the pollution caused by PCR products, and ensures the specificity and accuracy of amplification.
Kit application
Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.)
Workflow
Storage and Shelf life
This kit is stored at -20°C.
2× SuperEasyTM Mix(UNG)-EDTA，If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).
6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
