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SARS-CoV-2 Variant Nucleic Acid Detection Kit (I) (Multiplex PCR Fluorescent Probe Method)-for detection of mutants from Brazil,South Africa, and UK Featured Image
  • SARS-CoV-2 Variant Nucleic Acid Detection Kit (I) (Multiplex PCR Fluorescent Probe Method)-for detection of mutants from Brazil,South Africa, and UK

SARS-CoV-2 Variant Nucleic Acid Detection Kit (I) (Multiplex PCR Fluorescent Probe Method)-for detection of mutants from Brazil,South Africa, and UK

Short Description:

Kit Description: ◮ Direct PCR: No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.◮ Low equipment requirement and highly flexible use:Only need Real-Time PCR system .◮ LoD and high sensitivity:Detection sensitivity can reach the lowest level as 500 copies/ml.20+ years R&D Manufacturing Experience         25+ highest impact factor                       1000+ different lab reagents                                100+ universities partner                         400+ distributors                                                   2000+ SCI citations of the lab reagents


Product Detail

Product Tags

Specifications

48 Rxns/kit, 96 Rxns/kit

No.

Component

Amount

Main ingredient

48 Rxns

96 Rxns

1

Nucleic acid release agent

5.3 mL/ bottle

1 bottle

5.3 mL/bottle

2 bottle

Surfactant

2

RNA protectant

53 μL/tube

1 tube

106 μL/tube

1 tube

RNase inhibitor

3

SARS-CoV-2

reaction solution A

800 μL/tube

1 tube

1600 μL/tube

1 tube

Primer, probe, reaction buffer, dNTP

4

SARS-CoV-2

reaction solution B

800 μL/tube

1 tube

1600 μL/tube

1 tube

Primer, probe, reaction buffer, dNTP

5

SARS-CoV-2 enzyme mixture

160 μL/tube

1 tube

320 μL/tube

1 tube

Hot start Taq enzyme, M-MLV enzyme

6

SARS-CoV-2

positive control

100 μL/tube

1 tube

100 μL/tube

1 tube

Recombinant plasmid containing target fragment, RNA

7

SARS-CoV-2 negative control

1200 μL/tube

1 tube

1200 μL/tube

1 tube

TE buffer

Note:Components from different kits cannot be used interchangeably.

Features&advantages:

Quick and easy:complete the test in about 1 hour

Avoid nucleic acid loss:no need for nucleic acid purification

Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection

Low equipment requirement and highly flexible use

Detection sensitivity can reach the lowest level as 500 copies/mL).

How to use:

1.Applicable instrument

ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S

2.Detection procedure

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

Storage:

Sealed from light and stored at -20±5℃;

Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times)

Shelf life:1 year

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